美国罗莎琳德富兰克林医科大学微生物学与免疫学博士后职位
2014-01-21 09:50 来源: 未知 作者: liuxuehr
Postdoctoral Fellow
Rosalind Franklin University of Medicine and Science - Chicago, IL
The Department of Microbiology and Immunology at Rosalind Franklin University of Medicine and Science invites applications for a full-time Postdoctoral Fellow. The University encourages applications from individuals belonging to groups underrepresented in their field and those that can add new and valuable perspectives to the academic environment. The Postdoctoral Fellow will carry out advance laboratory research in biochemistry, cell biology, molecular biology and immunology relevant to photomedicine of Leishmania and leishmaniasis.Rosalind Franklin University of Medicine and Science - Chicago, IL
Rosalind Franklin University of Medicine and Science, a national leader in Interprofessional medical and healthcare education, is a graduate-level health sciences university situated on an attractive 85-acre campus in North Chicago, Illinois, approximately 40 miles north of the city of Chicago and 50 miles south of Milwaukee. As such, the University benefits from active collaborations with local hospitals, as well as academic institutions in both cities. The campus is shared with the North Chicago VA Medical Center and the Great Lakes Naval Training Center, both of which provide the University with potential research and clinical training resources.
Rosalind Franklin University is comprised of five Schools: the Chicago Medical School, College of Health Professions, Dr. William M. Scholl College of Podiatric Medicine, the College of Pharmacy and the School of Graduate and Postdoctoral Studies. Rosalind Franklin University enrolls approximately 2,000 students per academic year, and is supported by 850 full and part-time faculty and 338 staff members. Founded in 1912, Rosalind Franklin University has educated more than 16,000 medical and advanced health sciences degree graduates in the United States and worldwide.
Rosalind Franklin University of Medicine and Science promotes a dynamic learning environment in which qualified individuals of differing perspectives and cultural backgrounds pursue academic goals with mutual respect and shared inquiry.
The mission of Rosalind Franklin University is to serve the nation through the education of health professionals and the discovery of knowledge dedicated to improving the health of its people. Life in Discovery.
Essential Duties & Responsibilities
• Design plasmid constructs by PCR and molecular cloning.
• Produce transgenic Leishmania by using latest instrument for electroporation.
• Grow transgenic Leishmania in chemically defined medium of our own design.
• Use LumaCare illumination unit to calibrate the light intensity for cell photolysis.
• Assess cell viability by MTT assays based on metabolic activities in Biotek reader.
• Isolate and identify trangene products by mass spectrometry and proteomics.
• View infection of macrophages by confocal fluorescent microscopy and flow cytometry.
• Study signal pathways of infected cells by real-time cDNA and RT- PCR techniques.
• Develop high throughput fluorescent assays for screening effective compounds.
• cDNA seq. analyses of photolysed cells for transcriptional changes.
• Genetic knockout and knockdown by homologous recombination/antisense RNA .
• Nucleic acid sequence analysis for SNP mutation and genotyping.
Required Education & Experience
• Ph.D. degree.
• Research experiences along the lines mentioned above.
Required Knowledge, Skills and Abilities
• Excellent organizational skills.
• Knowledge about computer applications such as Word, Excel and PowerPoint.
• Excellent English reading, writing and verbal communication skills.
• Ability to collect and analyze data.
• Ability to work independently and as a team.
• Demonstrated project management skills, including ability set priorities and excellent time management skills.
• Ability to work productively with diverse populations; show respect and sensitivity for cultural differences.
Preferred Qualifications
• Cell cultivation/cryopreservation;
• Nucleic acid techniques;
• Genetic recombinant technology.
• Analytical protein chemistry; immunochemistry.
• Flow cytometry/confocal fluoresecent microscopy.
• Immune cell isolation, activation and activity analyses.
• Laboratory animal models for vaccination.
• Computation biology of DNA sequences;
• Presentation of seminar, reports, manuscripts and grant applications.
• Heme/porphyrin analytical chemistry.
Typical Physical Demands and Working Conditions
• Selected candidate must possess the mental and physical capability to perform the essential functions of the position with or without reasonable accommodations.
• Work is of a minimal physical demand in a laboratory setting with duties being performed while sitting, standing, or occasionally stooping with some movement and lifting of boxes with consumables.
• Some moderate levels of walking/lifting; experiences and training for safe-handling of biohazard/radioactive chemicals and biosafety level II pathogens (Leishmania).
• Work environment is normal laboratory environments for research in cell biology, biochemistry, molecular biology and immunology.
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