浙江大学医学院外籍教师Daniel H. Scharf实验室招聘博士后启事

Daniel H. Scharf团队正在招募博士后研究人员。

博士后岗位1：

项目背景 - 烟曲霉（Aspergillus fumigatus）是空气中普遍存在的腐生真菌之一。人类和动物不断大量吸入这种真菌的分生孢子。一般情况下，具有免疫能力的宿主会通过先天免疫机制将这些分生孢子清除。因此，A. fumigatus多年来被认为是一种弱病原体。然而在这些宿主中可以观察到两种不常见的感染性临床症状——曲霉瘤和过敏性支气管肺曲霉病。随着免疫抑制患者数量的增加，严重且通常是致命的曲霉病(目前世界上最常见的霉菌感染)的发生率急剧增加。我们实验室的研究重点包括（a）生物体的基因组和分子鉴定，（b）开发可用于诊断曲霉菌病的临床和实验室方法，（c）鉴定真菌在体内发生感染过程中起作用的宿主和真菌因子，（d）鉴定新的抗真菌靶标。

在这个项目中，应聘者加入我们团队后将使用我们先进的体外和离体模型系统来研究烟曲霉在致病过程中与宿主发生相互作用体系中的重要因子，重点研究烟曲霉与宿主上皮细胞的直接相互作用。总体目标是阐明导致宿主受损的致病机制。

应聘要求：应聘者已获得或即将获得生命科学方向的博士学位，例如生物学，生物化学或微生物学等。此外，应聘者应具有良好的团队协作精神以及独立开展研究工作的能力。具有微生物学，分子生物学，感染生物学，细胞生物学，细胞培养，基因组学或真菌遗传学等研究背景的优先。

博士后岗位2：

项目背景-胶质毒素Gliotoxin（GT）是ETP型真菌毒素的前体。 GT在烟曲霉的病理生物学中起关键作用。它能够调节免疫反应并诱导不同细胞类型的细胞凋亡。它的毒性归因于其分子内的一个特殊的二硫键，这个二硫键也是所有ETP的功能基序。具体而言，GT诱导细胞凋亡，通过抑制蛋白酶体抑制NF-B活化并抑制血管生成。这里仅列举GT对宿主细胞的几点作用。由各种致病真菌产生的其他ETP也可能在这些真菌的毒力机制中起作用。

在这个项目中，应聘者加入我们团队后将尝试鉴定和分离新的ETP，并致力于阐明这些ETP各自的生物合成途径。

应聘要求：应聘者已获得或即将获得博士学位，例如化学，生物学，生物化学或微生物学。此外，应聘者应具有良好的团队协作精神以及独立开展研究工作的能力。具有LC-MS，MS / MS，成像质谱（IMS），HPLC，NMR，HRMS或LC-MS等操作经验的应聘者优先。

博士后岗位3：

项目背景-胶质毒素Gliotoxin（GT）是ETP型真菌毒素的前体。 GT在烟曲霉的病理生物学中起关键作用。它能够调节免疫反应并诱导不同细胞类型的细胞凋亡。它的毒性归因于其分子内的一个特殊的二硫键，这个二硫键也是所有ETP的功能基序。具体而言，GT诱导细胞凋亡，通过抑制蛋白酶体抑制NF-B活化并抑制血管生成。这里仅列举GT对宿主细胞的几点作用。由各种致病真菌产生的其他ETP也可能在这些真菌的毒力机制中起作用。

该项目的目的是将天然产物生物合成途径的知识运用到有机化学方面。通过开发新型化学酶合成工具实现。在许多途径中，分子复杂性程度大部分是由酶的催化来实现的。根据这些酶在天然产物生物合成过程中是否能在关键步骤发挥生物催化剂的潜能，我们会进一步研究这类酶的特性。因此，我们将鉴定重要的酶并对其进行结构分析。

应聘要求：应聘者已获得或即将获得生命科学方向的博士学位，例如生物学，生物化学或微生物学等。此外，应聘者应具有良好的团队协作精神以及独立开展研究工作的能力。具有蛋白质表达和纯化，蛋白质结晶，晶体处理和X射线数据收集，蛋白质晶体学研究背景的应聘者优先。

基本要求

(1)优秀博士研究生，毕业于中国“双一流”大学或国外一流大学，年龄一般在35岁以下，外籍年龄放宽至40岁，身体健康。

(2)较强的科研创新能力和项目执行能力。

(3)能够全职从事博士后研究工作。

(4)以第一作者发表过SCI论文。

待遇：

1.按照浙江大学博士后管理的相关规定，薪酬待遇20-25万/年，并提供专业的科研指导和一流的科研环境。

2.博士后研究时间2-4年，由学校、导师、博士后本人根据研究项目确定。人事关系转入浙江大学从事博士后研究工作3年以上的，可以申请高级专业技术职务。

3.博士后研究员在研究期间可申请各类项目资助。

4.成果优秀、学术成绩突出的博士后研究人员，在出站时，可推荐其申请教师岗位。

所需材料：

应聘者请将中英文个人简历，以及已发表的文章，获奖证明等以压缩包的形式发至dscharf1@arcor.de，邮件主题为application for postdoctoral research fellows+name+name of supervisor+liuxuehr.com。

联系方式：

Dr. Daniel H. Scharf

E-mail: dscharf1@arcor.de

截止日期至招满为止

Dr. Daniel H. Scharf’s group is recruiting postdoctoral researchers.

Position 1：

Project background – Aspergillus fumigatus is one of the most ubiquitous of the airborne saprophytic fungi. Humans and animals constantly inhale numerous conidia of this fungus. The conidia are normally eliminated in the immunocompetent host by innate immune mechanisms, and aspergilloma and allergic bronchopulmonary aspergillosis, uncommon clinical syndromes, are the only infections observed in such hosts. Thus, A. fumigatus was considered for years to be a weak pathogen. With increases in the number of immunosuppressed patients, however, there has been a dramatic increase in severe and usually fatal invasive aspergillosis, now the most common mold infection worldwide. The focus of our lab, include (a) genomic and molecular characterization of the organism, (b) clinical and laboratory methods available for the diagnosis of aspergillosis in immunocompetent and immunocompromised hosts, (c) identification of host and fungal factors that play a role in the establishment of the fungus in vivo, and (d) identification of novel antifungal targets.

In this project, the successful applicant will use our sophisticated in vitro and ex vivo model systems to investigate important aspects of Aspergillus fumigatus interaction networks during pathogenesis, focusing on the direct interaction of A. fumigatus with host epithelial cells. The overarching goal is to elucidate the pathogenicity mechanisms which lead to host damage.

Candidate’s profile – We expect a degree in Life Sciences (e.g. Biology, Biochemistry, or Microbiology). Furthermore, the applicant should be able to perform team-oriented as well as independent work. Practical experiences in one or more of the following subjects are beneficial: Microbiology, Molecular Biology, Infection Biology, Cell Biology. Practical experience in cell culture, genomics or fungal genetics is an advantage.

Position 2：

Project background –Gliotoxin (GT) is the prototype of the epidithiodioxopiperazine (ETP)-type fungal toxins. GT plays a critical role in the pathobiology of Aspergillus fumigatus. It modulates the immune response and induces apoptosis in different cell types. The toxicity has been attributed to the unusual intramolecular disulfide bridge, which is the functional motif of all ETPs. Specifically, GT induces apoptosis, prevents NF-B activation by inhibition of the proteasome and inhibits angiogenesis, to mention only some of its effects on host cells. Other ETPs produced by various pathogenic fungi might also play a role in the virulence mechanisms of these fungi.

In this project, the successful applicant will try to identify and isolate novel ETPs and work on the elucidation of their respective biosynthetic pathways.

Candidate’s profile – We expect a degree in Life Sciences (e.g. Chemistry, Biology, Biochemistry, or Microbiology). Furthermore, the applicant should be able to perform team-oriented as well as independent work. Practical experiences in one or more of the following subjects are beneficial: hands-on skills in LC-MS and MS/MS, imaging mass spectrometry (IMS), previous experience in purification (HPLC) and chemical characterization of diverse types of natural products by high field NMR, HRMS, LC-MS.

Position 3：

Position Project background –Gliotoxin (GT) is the prototype of the epidithiodioxopiperazine (ETP)-type fungal toxins. GT plays a critical role in the pathobiology of Aspergillus fumigatus. It modulates the immune response and induces apoptosis in different cell types. The toxicity has been attributed to the unusual intramolecular disulfide bridge, which is the functional motif of all ETPs. Specifically, GT induces apoptosis, prevents NF-B activation by inhibition of the proteasome and inhibits angiogenesis, to mention only some of its effects on host cells. Other ETPs produced by various pathogenic fungi might also play a role in the virulence mechanisms of these fungi.

The aim of the project is to translate the knowledge from biosynthetic pathways of natural products into organic chemistry, achieved by developing novel chemoenzymatic synthesis tools. In many pathways large parts of the molecular complexity are set up by tailoring enzymes. These enzymes will be studied regarding their potential to act as biocatalysts that can substitute late and crucial steps in total syntheses. Therefore, interesting candidate enzymes should be identified and structural characterized.

Candidate’s profile – We expect a degree in Life Sciences (e.g. Biology, Biochemistry, or Microbiology). Furthermore, the applicant should be able to perform team-oriented as well as independent work. Practical experiences in one or more of the following subjects are beneficial: protein expression, purification and characterization, hands-on experience in protein crystallization, crystal handling and X-ray data collection, experience in protein crystallography including data processing, structure determination and refinement is a plus.

Requirements

1. Basic requirements

The ideal candidates would be or possess:

（1） Outstanding newly PhD graduates from top overseas universities or universities listed in China’s “Double-First Class” initiative or preponderant disciplines, usually under 35 years old for Chinese applicant, 40 years old for Foreign applicant, and healthy.

（2） Strong capability of research innovation and project organization.

（3） Available for full-time post-doctoral research work.

2. Academic requirements

The candidates are supposed to meet the academic requirements of the School of Medicine, Zhejiang University

Package

1. The annual salary is around RMB200, 000 yuan, and the fringe benefits are subject to the relevant post-doctoral regulations of Zhejiang University.

2. The post-doctoral research lasts from 2 to 4 years determined by the school, supervisor, and postdoctoral fellow himself according to the research projects. Postdoctoral research fellows who have been in the position for 3 years or above may apply for senior professional and technical positions.

3. Postdoctoral research fellows can apply for various projects funding during their stay.

4. Postdoctoral research fellows with excellent performance and outstanding academic achievements can be recommended to apply for the position of teachers in the school when they leave the position.

Application materials

1. Resume

2. Academic achievements and rewards list

3. Supporting materials

Please submit the application materials to Dr. Daniel H. Scharf：dscharf1@arcor.de with the subject “application for postdoctoral research fellows+name+name of supervisor+liuxuehr.com”.

Contact information

Dr. Daniel H. Scharf

E-mail: dscharf1@arcor.de 

原文出处：http://hr.zju.edu.cn/postdoctor/redir.php?catalog_id=68088&object_id=109104